Mechanisms of inhibition of triacylglycerol hydrolysis by human gastric lipase.
Identifieur interne : 002157 ( Main/Exploration ); précédent : 002156; suivant : 002158Mechanisms of inhibition of triacylglycerol hydrolysis by human gastric lipase.
Auteurs : Yan Pafumi [France] ; Denis Lairon ; Paulette Lechene De La Porte ; Christine Juhel ; Judith Storch ; Margit Hamosh ; Martine ArmandSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Lipase.
- chemical , metabolism : Lipase, Triglycerides.
- chemical : Emulsions.
- enzymology : Gastric Juice.
- physiology : Lipolysis.
- Adult, Homeostasis, Humans, Hydrolysis, Kinetics.
Abstract
In the human stomach, gastric lipase hydrolyzes only 10 to 30% of ingested triacylglycerols because of an inhibition process induced by the long chain free fatty acids generated, which are mostly protonated at gastric pH. The aim of this work was to elucidate the mechanisms by which free fatty acids inhibit further hydrolysis. In vitro experiments examined gastric lipolysis of differently sized phospholipid-triolein emulsions by human gastric juice or purified human gastric lipase, under close to physiological conditions. The lipolysis process was further investigated by scanning electron microscopy, and gastric lipase and free fatty acid movement during lipolysis were followed by fluorescence microscopy. The results demonstrate that: 1) free fatty acids generated during lipolysis partition between the surface and core of lipid droplets with a molar phase distribution coefficient of 7.4 at pH 5.40; 2) the long chain free fatty acids have an inhibitory effect only when generated during lipolysis; 3) inhibition of gastric lipolysis can be delayed by the use of lipid emulsions composed of small-size lipid droplets; 4) the release of free fatty acids during lipolysis induces a marked increase in droplet surface area, leading to the formation of novel particles at the lipid droplet surface; and 5) the gastric lipase is trapped in these free fatty acid-rich particles during their formation. In conclusion, we propose a model in which the sequential physicochemical events occurring during gastric lipolysis lead to the inhibition of further triacylglycerol lipolysis.
DOI: 10.1074/jbc.M202839200
PubMed: 11940604
Affiliations:
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last="Lairon">Denis Lairon</name></author><author><name sortKey="De La Porte, Paulette Lechene" sort="De La Porte, Paulette Lechene" uniqKey="De La Porte P" first="Paulette Lechene" last="De La Porte">Paulette Lechene De La Porte</name></author><author><name sortKey="Juhel, Christine" sort="Juhel, Christine" uniqKey="Juhel C" first="Christine" last="Juhel">Christine Juhel</name></author><author><name sortKey="Storch, Judith" sort="Storch, Judith" uniqKey="Storch J" first="Judith" last="Storch">Judith Storch</name></author><author><name sortKey="Hamosh, Margit" sort="Hamosh, Margit" uniqKey="Hamosh M" first="Margit" last="Hamosh">Margit Hamosh</name></author><author><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11940604</idno><idno type="pmid">11940604</idno><idno 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Méditerranée, 18 avenue Mozart, 13009 Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité 476-INSERM (National Institute of Health and Medical Research)/Université de la Méditerranée, 18 avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation></author><author><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name></author><author><name sortKey="De La Porte, Paulette Lechene" sort="De La Porte, Paulette Lechene" uniqKey="De La Porte P" first="Paulette Lechene" last="De La Porte">Paulette Lechene De La Porte</name></author><author><name sortKey="Juhel, Christine" sort="Juhel, Christine" uniqKey="Juhel C" first="Christine" last="Juhel">Christine Juhel</name></author><author><name sortKey="Storch, Judith" sort="Storch, Judith" uniqKey="Storch J" first="Judith" last="Storch">Judith Storch</name></author><author><name sortKey="Hamosh, Margit" sort="Hamosh, Margit" uniqKey="Hamosh M" first="Margit" last="Hamosh">Margit Hamosh</name></author><author><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="ISSN">0021-9258</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Emulsions</term><term>Gastric Juice (enzymology)</term><term>Homeostasis</term><term>Humans</term><term>Hydrolysis</term><term>Kinetics</term><term>Lipase (antagonists & inhibitors)</term><term>Lipase (metabolism)</term><term>Lipolysis (physiology)</term><term>Triglycerides (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Lipase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lipase</term><term>Triglycerides</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Emulsions</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Gastric Juice</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lipolysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Homeostasis</term><term>Humans</term><term>Hydrolysis</term><term>Kinetics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the human stomach, gastric lipase hydrolyzes only 10 to 30% of ingested triacylglycerols because of an inhibition process induced by the long chain free fatty acids generated, which are mostly protonated at gastric pH. The aim of this work was to elucidate the mechanisms by which free fatty acids inhibit further hydrolysis. In vitro experiments examined gastric lipolysis of differently sized phospholipid-triolein emulsions by human gastric juice or purified human gastric lipase, under close to physiological conditions. The lipolysis process was further investigated by scanning electron microscopy, and gastric lipase and free fatty acid movement during lipolysis were followed by fluorescence microscopy. The results demonstrate that: 1) free fatty acids generated during lipolysis partition between the surface and core of lipid droplets with a molar phase distribution coefficient of 7.4 at pH 5.40; 2) the long chain free fatty acids have an inhibitory effect only when generated during lipolysis; 3) inhibition of gastric lipolysis can be delayed by the use of lipid emulsions composed of small-size lipid droplets; 4) the release of free fatty acids during lipolysis induces a marked increase in droplet surface area, leading to the formation of novel particles at the lipid droplet surface; and 5) the gastric lipase is trapped in these free fatty acid-rich particles during their formation. In conclusion, we propose a model in which the sequential physicochemical events occurring during gastric lipolysis lead to the inhibition of further triacylglycerol lipolysis.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université de la Méditerranée</li></orgName></list><tree><noCountry><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name><name sortKey="De La Porte, Paulette Lechene" sort="De La Porte, Paulette Lechene" uniqKey="De La Porte P" first="Paulette Lechene" last="De La Porte">Paulette Lechene De La Porte</name><name sortKey="Hamosh, Margit" sort="Hamosh, Margit" uniqKey="Hamosh M" first="Margit" last="Hamosh">Margit Hamosh</name><name sortKey="Juhel, Christine" sort="Juhel, Christine" uniqKey="Juhel C" first="Christine" last="Juhel">Christine Juhel</name><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name><name sortKey="Storch, Judith" sort="Storch, Judith" uniqKey="Storch J" first="Judith" last="Storch">Judith Storch</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Pafumi, Yan" sort="Pafumi, Yan" uniqKey="Pafumi Y" first="Yan" last="Pafumi">Yan Pafumi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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